This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 ° C) until the bottom phase (phenol) becomes clear.
It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions, and a carbohydrate as … Does anyone have any suggestion for tris-buffered phenol The tris is used to buffer phenol to insure that the "aqueous compartment" is at a suitable pH for what you are doing. Why is phenol saturated with water or tris HCl (pH 8) for It involves repeated extraction of the cells, suspended in buffered 1 M NaCl, with water-saturated phenol. The product obtained is remarkably free of contamination by polypeptides, polysaccharides Learning Center - Phenolic Solutions: Are They Cleaners or
HBSS with 10 mM HEPES, Without Phenol Red
Buffer-Saturated Phenol UltraPure Buffer-Saturated Phenol grade of phenol by repeated extraction with aqueous Tris-HCl buffer (pH = 8.0). The final product has a pH ≥ 7.4 at 25°C. It is free from interfering levels of heavy metals and antioxidants, including hypophosphorous acid. UltraPure™ Buffer-Saturated Phenol is a ready-to-use solution, suitable for phenol extraction of nucleic acid solutions. Acid Phenol Chloroform Extraction of DNA, RNA and protein Nov 23, 2016
HBSS with 10 mM HEPES, Without Phenol Red
buffer (Sigma Chemical Co., Poole, Dorset, England) and 3.5 x 10~" M testosterone but otherwise as for the human cells above. Growth Curves in Monolayer Culture. Cells from stock plates were washed in phosphate-buffered saline to remove all phenol red, sus pended by treatment in a 0.06% trypsin/0.02% EDTA, pH 7.3, solution Spectrophotometric Determination of p Ka of phenol red